Management of Biofilm with Breast Implant Surgery | Journal CME Article-Journal CME Article: Management of Biofilm with Breast Implant Surgery Video 6 of 7

Journal CME Article: Management of Biofilm with Breast Implant Surgery Video 6 of 7

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Video Summary

A multimodal approach effectively identifies bacteria on implant surfaces, surrounding fluids, and tissues. Techniques like light and laser scanning microscopy, and infrared imaging assess bacteria abundance and orientation. Scanning electron microscopy offers detailed imaging but may face sampling biases. Standard and sonicated cultures are common, with the latter dislodging biofilms for better analysis. Genomic advancements enable bacterial identification via DNA sequencing, using methods such as Sanger sequencing, 16S RNA, and shotgun metagenomic sequencing. Proteomics, transcriptomics, and metabolomics further explore bacterial functions and interactions with hosts through various advanced techniques like mass spectrometry and x-ray crystallography.

Keywords

multimodal approach

bacterial identification

microscopy techniques

genomic advancements

proteomics and metabolomics